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The IGF-1 axis benefits from the same pause: it drifts back toward baseline instead of sitting chronically elevated, which is the safer position given GH/IGF-1s growth-promoting signaling

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Subcutaneous injection (under the skin) is the most effective delivery method

However, implementation of eryptosis assays in nanotoxicology can be limited by the lack of a generally accepted single eryptosis marker and the requirements to use several markers simultaneously, their lower cost-effectiveness compared to the hemolysis assays, and the availability of the equipment, since eryptosis detection is primarily flow cytometry-based